In Vitro Antioxidant Activity, Proximate Composition And Phytochemical Composition Of Hibiscus Sabdariffa.

ABSTRACT

Hisbiscus S'abdariffà is known for delicacy and also for medicinal properties. The present work aim to study the phytochemical, proximate and antioxidant screening of' J-Iisbisczi.s ,solvluii/fu for various medicinally important compound and their quantification. i'he result showed that calcium. niacin, riboflavin, iron, phosphorous, and protein are present in calyces of hisbisbus sabdari/Ja. Protein content was highest while the contents of calcium, niacin, riboflavin, iron, phosphorous were lowest. The major compounds are gossypetin, hibiscetin, quercetin and sabdarefin (Flavonoids) while delphinidin 3-sambubioside and cyaniding 3-sambudioside are the major anthocyanins. The antioxidant activity of the investigated extract has a scavenging ability of DPPH (517nm). The IC5O value of Hsbiscus andari/fa extract was I OOug/ml while that of ascorbic acid used the reference control was 6.7mg. This indicated that the compounds consisting many calyces of Hisbiscus Sabdarif/i.such as anthocanins. fiavonoids, phenolic acid contribute to the antioxidant activity. Findinds provide evidence that the calyces extract of ilishiscus Sc'bdcu'i//a is a potential source of natural antioxidant. This justify its use in folkloric medicines.