EVALUATION OF MICROORGANISMS FROM GARI AT SELECTED MARKETS IN IKWUANO LGA OF ABIA STATE

ABSTRACT

Gari sold in Ndoru and Gate 6 market were examined with the aim of investigating the bacterial contamination of garri due to exposure in the two markets as well as establish their hygienic statues. To achieve this, a total of 20 samples were collected from Ndoru and Gate 6 market. The samples were analyzed bacteriologically for reliable heterotrophic bacteria and coliform bacteria counts on nutrient and MacConkey agar respectively. Using a pour plate method, E. coli showed a high percentage prevalence of 50%  from red gari samples obtained  from Ndoru market while Mucor Spp showed a high percentage from the white garri sample obtained  from Ndoru market. E. coli  and Mucor spp 50% respectively showed a high percentage from the red garri sample bought from Gate 6 market while Bacillus 35% showed a high percentage prevalence among the bacterial isolates. Hence, appropriate authority is advised to take measures to ensure that food             for public consumption are prepared under hygienic condition.   

TABLE OF CONTENTS

Title Page ﾿ i

Certification ﾿ ii

Dedication ﾿ iii

Acknowledgement                                              ﾿ iv

Table of Contents        ﾿ v

List of Tables ﾿ vii

Abstract                                ﾿ viii

CHAPTER ONE

1.0 ﾿ INTRODUCTION ﾿ 1

1.1 AIMS AND OBJECTIVES ﾿ 2

1.2 SIGNIFICANCE OF STUDY ﾿ 2

CHAPTER TWO

2.0 ﾿ LITERATURE REVIEW ﾿ 4

2.1 ﾿ CASSAVA ﾿ 4

2.1.1 ﾿ Pests and Potential Problems ﾿ 5

2.2 ﾿ MICROBES ASSOCIATED WITH FOOD ITEMS ﾿ 7 

2.3 ﾿ FOOD POISON ﾿ 8

2.3.1 ﾿ Prevention of Food Poisoning ﾿ 15

CHAPTER THREE

3.0 ﾿ MATERIALS AND METHODS ﾿ 16

3.1 ﾿ SOURCE OF SAMPLE ﾿ 16

3.2 ﾿ SAMPLING PREPARATION ﾿ 16

3.3 ﾿ MEDIA USED ﾿ 16

3.4 ﾿ STERILIZATION ﾿ 16

3.5  IDENTIFICATION OF BACTERIAL ISOLATES ﾿ 17

3.6 ﾿ GRAM STAINING ﾿ 16

3.7    CATALASE TEST ﾿ 17

3.7.1 ﾿ Coagulase Test ﾿ 18

3.7.2 ﾿ Citrate Test ﾿ 18

3.7.3 ﾿ Motility, Indole, Urease Test (MIU). ﾿ 18

3.7.4 ﾿ Triple Sugar Iron Test ﾿ 19

3.7.5 ﾿ Oxidase Test ﾿ 19 ﾿

CHAPTER FOUR 

4.0 ﾿ RESULTS ﾿ 20

CHAPTER FIVE 

5.0 ﾿ DISCUSSION, CONCLUSION AND RECOMMENDATION

5.1 ﾿ Discussion  ﾿ 25

5.2 ﾿ Conclusion ﾿ 26

5.3 ﾿ Recommendation ﾿ 27

References ﾿ 28