Cyanogenic Potential And Activity Of Partially Purified B-Glucosidase Of Cassava Cultivar TMS 1089A

ABSTRACT

This work aimed to determine the cyanogenic potential of cassava

cultivar TMS 1089A, using the protocol- B method and also the activity

of the partially purified enzyme which was extracted from the cassava

peel using ammonium sulphate precipitation method. The result of the

analysis showed that a higher total cyanide distribution was found in the

dried leaves of the cassava cultivar with 268.092ppm, followed by the

fresh peel (138.204ppm), fresh leaves (79.990ppm), fresh stem

(43.560ppm), fresh pulp(29.700ppm), dried pulp (17.820ppm) and then

the dried peel (5.148ppm) which has the lowest cyanide content. The

partially purified enzyme has an activity of 4.Ounits/ml and specific

activity of O.63units/mg protein. The analysis for Carotenoid gave a

value of 6.514mgJg and protein content was 6.36mg/ml. The partially

purified enzyme showed high degree of hydrolytic activity towards the

cyanogenic glycosides of cassava and products of some other root tubers

than when compared to 3-glucosidase of activity 3.Ounit/ml.