Characterization And Antibiotic Susceptibility Profile Of Bacterial Isolates In Urine Samples From Michael Okpara University Of Agriculture Students:- Azubuike, Chioma S

ABSTRACT

Various microorganisms are able to invade the urinary tract and can be involved in the

pathogenesis of urinary tract infection. The isolation and identification of bacteria species

from urine samples of Michael Okpara University of Agriculture students (MOUAU)

students and determination of its antibiotics sensitivity were carried out. This revealed that

out of forty-five (45) urine samples examined from the male and female students of

MOUAU, thirty four (34) showed positive growths at 73.3% frequency occurrence, while

eleven (11) samples showed negative cultures at 24.4% frequency occurrence respectively. It

was also revealed that a total of five (5) bacteria species were isolated and identified by their

morphological characteristics, pigmentation on media, microscopy, and biochemical

methods. The bacterial species isolated from the urine samples include Escherichia coli,

Klebsiella spp. Staphylococcus aureus, Proteus spp and Coagulase Negative Staphylococcus

aureus respectively. The distribution and percentage occurrence of bacterial isolates from the

urine specimens were different. In the urine specimens from female individuals, the

predominant bacterial isolates were Staphylococcus aureus 19(35.8%), followed by

Escherichia coli 17(32.1%) and Klebsiella spp 11(20.7%), while in male individuals the

predominant bacterial isolates were Staphylococcus aureus 11(34.3%), followed by

Escherichia coli 9(28.1%) and Klebsiella spp 6(18.8%). It was also revealed in this study that

the antibiotic susceptibility and resistant profile for bacteria isolates varied. Escherichia coli

and Staphylococcus aureus showed high level of sensitive to the antibiotics Rifampicin

(20mcg), Ciprofloxacin (lOmcg), Ampiclox (20mcg), and Gentamycin (lOmcg), while the

resistant profile of the isolates revealed that Escherichia coli and Staphylococcus aureus had

highest number of isolates resistant to the antibiotic drug Rifampicin (20mcg) and

Chloramphenicol (30mcg). This implies that these antibiotics cannot be used as empirical

therapy for urinary tract infection particularly in the study area. However, Rifampicin

(20mcg), Ciprofloxacin (lOmcg), Ampiclox (20mcg), and Gentamycin (lOmcg) antibiotic

were the most effective drug against urinary tract infections, thus could be of alternative of

choice to use and to control bacterial infection as an effective antibacterial agent.