Antimicrobial Activity Of Helianthus Annuus Leaves On Some Bacterial Pathogens (E.Coli, Salmonella Spp, Shigella Spp, And Staphylococcus Aureus)

ABSTRACT

The antibacterial activity of Helianthus annuus leaves on some bacterial pathogens (E. coil, Salmonella spp, Shigella spp, and Staphylococcus aureus) was evaluated. The extracts were prepared using chloroform, N-hexane and methanol. The antibacterial susceptibility testing of the extracts were carried out using the Agar well diffusion method. The phytochemical analysis was done using standard method while the antioxidant activity was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) photometric assay. The antibacterial testing showed that the test organism were all susceptible to the extract. S. aureus showed the highest susceptibility to the extracts while Shigella spp showed least susceptibility. The Minimum inhibitory concentration of the methanolic fraction of the extract was determined to be 125tgIml on all the test organisms. The phytochemical analysis show the presence of Saponins, Tannins, Glycoside, Alkaloids and Flavonoids. The extracts produced a concentration dependent increase in antioxidant activity. The chloroform showed greater antioxidant activity while the methanolic extract was least compared to the standard solution or Ascorbic acid. This therefore gives a scientific justification for the use of Helianthus annus leave on some bacterial pathogens