Microbial Quality And Antibiotics Sensitivity Pattern Of Gram Negative Organisms Of Public Health Importance Isolated From Soymilk

Authors: EGEMONU MARTINS CHIGOZIE. | Natural & Applied Sciences Microbiology Projects 51 pages 9,271 words

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ABSTRACT

The microbial quality and Antibiotics sensitivity, pattern of Gram negative organisms of public health importance was investigated, the organisms isolated were E.coli, Salmonella, Si apinocuccus, Laciobacillus, Kiebsiella, Bacillus and Shigella. The microbiological count includes total viable count on the nutrient agar (NA) ranging between 3 .8x 10 and 4.9xl0 the total coliform count on MacConkey agar (MA) ranged from 0.7x105to 6.3x105, the total E.coli count w LMB range from 1.1 x105to 2.7x1 while the total coliform count on Salmonella Shigelln agar (SSA) ranged from 0.4x105to 4.6x105. The antibiotics sensitivity test was done using disc method containing multiple antibiotics that includes gentalnicin, ceftazidinc, cefuioximc, ceftriaxonc, erythromycin, cloxacillin, ofloxacin, and augumentin were used inthe antibiotics sensitivity.olloxacin showed 8(80%) sensitive to the organism, gentamicine showed 2(20) sensitive, 5(50%) intermediate and 3(30%) resistance. While the other antibiotics ised showed 10(1 00%) resistance as there is no zone of inhibition in the media. This means that olloxaein amongst all antibiotics used, is the most effective in the treatment of infection caused by Ecoli. The result of the antibiotics sensitivity shows that all the isolates were resistance to; the other antibiotics used and only ofioxacin was sensitive to Ecoli as they can be qualified as extended spectrum Beta lactamase producers.

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