In-Vitro Synergistic Antibacterial Effect Of Aqueous And Ethanolic Extract Of Garcinia Kola Seeds And Gongmnema Lahfolium Leaves On Some Selected Enteric Pathogens.

ABSTRACT

The in-vitro synergistic antibacterial effect of aqueous and ethanolic extract of Gongronema latfolium leaves and Garcinia kola seeds investigated against Siaphylococcus aureus, Pseudomonas aeruginosa and Escherichia coil. Agar well diffusion method was used for the antibacterial activity. The susceptibility of the test organisms to the plant extracts was performed and diameters of the inhibition were recorded. The ethanolic extract of the two plants in synergy showed the highest inhibitory activity. P. aeruginosa was the most susceptible (23.3mm) to the extract in synergy while S. aureus was inhibited the least (6.3mm) for the aqueous extract and was compared to standard control ciprofloxacin. The minjmum inhibitory concentration (MIC) was evaluated for each plant extract and ranged from 6.25 to 100mg/mi. The result of this research work showed that the two plants in synergy had higher antibacterial activity compared to the plant when used individually. This can be employed as a form of antibacterial agent.